Long term betaine supplementation regulates genes involved in lipid and cholesterol metabolism of two muscles from an obese pig breed

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• 作者：A. Albuquerque^a ; José ; A. Neves^b ; M. Redondeiro^a ; M. Laranjo^a ; M.R. Fé ; lix^c ; Amadeu Freitas^b ; José ; L. Tirapicos^d ; José ; M. Martins^b ; ^{jmartins@uevora.pt}
• 关键词：ACC ; Acetyl-CoA carboxylase ; ADPN ; adiponectin ; AL ; Alentejano ; BW ; body weight ; C ; commercial ; CB ; C diet supplemented with betaine ; FA ; fatty acid ; FASN ; Fatty acid synthase ; H-FABP ; Heart-type fatty acid binding protein ; HMGCR ; 3-Hydroxy-3-methyl-glutaryl-CoA reductase ; HPRT1 ; hypoxanthine phosphoribosyltransferase 1 ; HSL ; Hormone-sensitive lipase ; IMF ; intramuscular fat ; LDLr ; Low-Density Lipoprotein Receptor ; LPL ; Lipoprotein lipase ; MCPT1 ; Muscle-type carnitine palmitoyltransferase 1 ; MUFA
• 刊名：Meat Science
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：124
• 期：Complete
• 页码：25-33
• 全文大小：418 K
• 卷排序：124

文摘

This study evaluates the effects of betaine supplementation (1 g kg− 1 for 20 weeks) on the regulation of genes involved in lipid and cholesterol metabolism of Longissimus lumborum and Biceps femoris from obese Alentejano pigs. Betaine supplementation led to an increase in total cholesterol in both muscles, complementing results previously published indicating a significant increase on the intramuscular lipid content. The expression of twelve genes involved in lipogenesis, lipolysis/FA oxidation, FA transport, and cholesterol metabolism, as well as two transcription factors were also evaluated. Genes related to lipid and cholesterol synthesis plus FA transport were consistently up-regulated in both muscles of betaine fed pigs. On the other hand, genes related to lipolysis/FA oxidation were not affected or down-regulated by betaine supplementation.Our data suggest that the underlying mechanism regulating IMF and cholesterol accumulation in Alentejano pigs supplemented with betaine is associated with the up-regulation of genes involved in lipid synthesis, FA transport, and cholesterol synthesis.