All trans retinoic acid depresses the content and activity of the mitochondrial ATP synthase in human keratinocytes

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• 作者：F. Papa^a ; R. Lippolis^b ; N. Sardaro^a ; A. Gnoni^a ; S. Scacco^a ; ^{salvatore.scacco@uniba.it}
• 关键词：Human keratinocytes ; All-trans retinoic acid ; ATP synthase ; Mass spectrometry ; Proteomics
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：8 January 2017
• 年：2017
• 卷：482
• 期：2
• 页码：301-304
• 全文大小：822 K
• 卷排序：482

文摘

Proteomic analysis shows that treatment of keratinocytes cultures with all trans retinoic acid (ATRA), under condition in which it inhibits cell growth, results in marked decrease of the level of the F1-β subunit of the catalytic sector of the mitochondrial FoF1 ATP synthase complex. Enzymatic analysis shows in ATRA-treated keratinocytes a consistent depression of the ATPase activity, with decreased olygomycin sensitivity, indicating an overall alteration of the ATP synthase complex. These findings, together with the previously reported inhibition of respiratory complex I, show that depression of the activity of oxidative phosphorylation enzymes is involved in the cell growth inhibitory action of ATRA.