High-virulence CMY-2- and CTX-M-2-producing avian pathogenic Escherichia coli strains isolated from commercial turkeys

详细信息    查看全文

• 作者：Ketrin Cristina da Silva^a ; Marcos Paulo Vieira Cunha^b ; Louise Cerdeira^c ; Maria Gabriela Xavier de Oliveira^b ; Mirela Caroline Vilela de Oliveira^b ; Cleise Ribeiro Gomes^a ; Nilton Lincopan^c ; ^d ; Terezinha Knö ; bl^b ; Andrea Micke Moreno^a ; ^{morenoam@usp.br}
• 关键词：CMY-2 ; Turkey ; Plasmid ; IncI1/ST12 ; ESBL
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：87
• 期：1
• 页码：64-67
• 全文大小：453 K
• 卷排序：87

文摘

This study reports the high-virulence phylogenetic backgrounds of CMY-2- and CTX-M-2-producing avian pathogenic Escherichia coli strains isolated from turkeys sent to slaughter and condemned by airsacculitis in Brazil. Among 300 air sac samples, seven E. coli strains produced plasmid-mediated CMY-2-type AmpC, of which three carried also the blaCTX-M-2 Extended Spectrum Beta-Lactamase encoding gene. Interestingly, the transfer of the blaCMY-2 gene was positive for three E. coli strains, being associated with the presence of IncI1 plasmids. The complete sequence of the representative pJB10 plasmid revealed that the blaCMY-2 gene was within a transposon-like element in the classical genetic environment consisting of tnpA-blaCMY-2-blc-sugE structure. This plasmid with 94-kb belonged to the sequence type (ST) 12 among IncI1 plasmids, which has been associated with the worldwide spread of blaCMY-2 among Salmonella enterica and E. coli. Furthermore, to the best of our knowledge, this is the first complete sequence of a CMY-2-encoding plasmid derived from an Escherichia coli isolated from food-producing animals in Latin America.