TCDD, the most potent congener of the polychlorinated dioxins, has been shown to be an antiestrogen. The mechanisms of TCDD-induced antiestrogenicity are still under investigation. In this study, we investigated the effects of TCDD on the expression of the estrogen receptor (ER) gene. We studied the levels of un-spliced ER transcript (hnRNA) as well as the ER mRNA in ovary, uterus and liver of TCDD-treated mice with different genetic backgrounds. To quantitate the ER hnRNA levels, the intron and exon boundary of ER hnRNA was amplified by competitive RT-PCR. The ER mRNA from these mice was quantitated by competitive RT-PCR amplifying exons separated by an intron. ER hnRNA and ER mRNA levels were quantitated 4days after a single i.p. dose of TCDD (5
μg/kg) in female C57BL/6J (B6) mice, which carry the responsive allele to TCDD. TCDD treatment significantly (
p<0.05) suppressed the levels of ER hnRNA in the ovary (27.4 % ) and uterus (21.9 % ). The decreases in ER hnRNA were coordinated with significant (
p<0.01) decreases in ER mRNA in ovary (57.7 % ) and uterus (37.6 % ). There was a significant decrease (20.3 % ,
p<0.05) in liver ER mRNA, however, the changes of ER hnRNA in liver were not significant. The coordinated decreases in ER hnRNA and mRNA in TCDD-treated mice suggest a suppression of transcription of the ER gene. We performed the same study on DBA/2J (D2) mice, which possess the “non-responsive” allele of the aryl hydrocarbon receptor (AhR). These mice demonstrated no significant decrease in either the ER mRNA or hnRNA after TCDD treatment. Overall, these results suggest that TCDD suppresses the gene expression of the ER receptor by decreasing its transcription, and the AhR plays an important role in mediating this response.
Publisher: | Elsevier Science |
Language of Publication: | English |
Item Identifier: | S0960-0760(98)00067-3 |
Publication Type: | Article |
ISSN: | 0960-0760 |
Cited by: | - Dasmahapatra, Asok K.; Wimpee, Barbara A.B.; Trewin, Amanda L.; Hutz, Reinhold J.,""2,3,7,8-Tetrachlorodibenzo-p-dioxin increases steady-state estrogen receptor-b mRNA levels after CYP1A1 and CYP1B1 induction in rat granulosa cells in vitro1""Molecular and Cellular Endocrinology2001pp. 39-48
Bibliographic PageFull Text - Haavisto, Tapio; Nurmela, Katri; Pohjanvirta, Raimo; Huuskonen, Hannele; El-Gehani, Faraj; Paranko, Jorma,""Prenatal testosterone and luteinizing hormone levels in male rats exposed during pregnancy to 2,3,7,8-tetrachlorodibenzo-p-dioxin and diethylstilbestrol""Molecular and Cellular Endocrinology2001pp. 169-179
i1 -2&article=169_ptalhldpt2ad"">Bibliographic Pagei1-2&article=169_ptalhldpt2ad&form=pdf&file=file.pdf"">Full TextFujise, Hiroshi; Annoura, Takeshi; Sasawatari, Shigemi; Ikeda, Teruo; Ueda, Kazumitsu,""Transepithelial transport and cellular accumulation of steroid hormones and polychlorobiphenyl in porcine kidney cells expressed with human P-glycoprotein""Chemosphere2002pp. 1505-1511 Bibliographic PageFull TextMitsushima, Dai; Funabashi, Toshiya; Kimura, Fukuko,""Estrogen increases messenger RNA and immunoreactivity of aryl-hydrocarbon receptor nuclear translocator 2 in the rat mediobasal hypothalamus""Biochemical and Biophysical Research Communications2003pp. 248-253 Bibliographic PageFull TextCrouse, Karen A.; Chew, Kar-Beng; Tarafder, M.T.H.; Kasbollah, A.; Ali, A.M.; Yamin, B.M.; Fun, H.-K.,""Synthesis, characterization and bio-activity of S-2-picolyldithiocarbazate (S2PDTC), some of its Schiff bases and their Ni(II) complexes and X-ray structure of S-2-picolyl-b-N-(2-acetylpyrrole)dithiocarbazate""Polyhedron2004pp. 161-168 Bibliographic PageFull TextKlinge, Carolyn M.; Kaur, Kulwant; Swanson, Hollie I.,""The Aryl Hydrocarbon Receptor Interacts with Estrogen Receptor Alpha and Orphan Receptors COUP-TFI and ERRa1""Archives of Biochemistry and Biophysics2000pp. 163-174 tahriwaorcae"">Bibliographic Pagetahriwaorcae&form=pdf&file=file.pdf"">Full Text |
| Footnotes: Part of the work communicated in this paper was presented in the 82nd (Toronto, Canada) Annual Meetings of the Endocrine Society. All cDNA clones used in the present experiments are available from the laboratory of Professor Hutz.
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