Sphingolipid C4 hydroxylation influences properties of yeast detergent-insoluble glycolipid-enriched membranes

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• 作者：Idkowiak-Baldys ; Jolanta ; Grilley ; Michelle M. ; Takemoto ; Jon Y.
• 关键词：Syringomycin E ; Lipid raft ; Sphingolipid ; SUR2 ; Saccharomyces cerevisiae
• 刊名：FEBS Letters
• 出版年：2004
• 出版时间：July 2, 2004
• 年：2004
• 卷：569
• 期：1-3
• 页码：272-276
• 全文大小：256 K

文摘

Sphingoid base C4 hydroxylation is required for syringomycin E action on the yeast plasma membrane. Detergent-insoluble glycolipid-enriched membranes (DIGs) from a yeast strain lacking C4 hydroxylated sphingoid bases (sur2Δ) are composed of linear membrane fragments instead of vesicular structures observed for wild-type DIGs, though they have similar lipid compositions and amounts of DIG marker proteins. Light-scattering bands collected from sur2Δ after centrifugation of Triton X-100-treated cell lysates in continuous density gradients have lower buoyant densities than that of the wild-type. The results show that C4 hydroxylation influences the physical and structural properties of DIGs and suggest that syringomycin E interacts with lipid rafts.