Multi-laboratory survey of qPCR enterococci analysis method performance in U.S. coastal and inland surface waters
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- 作者：Richard A. Hauglanda ; haugland.rich@epa.gov" class="auth_mail" title="E-mail the corresponding author ; Shawn Siefringa ; Manju Varmaa ; Kevin H. Oshimaa ; Mano Sivaganesanb ; Yiping Caoc ; Meredith Raithc ; John Griffithc ; Stephen B. Weisbergc ; Rachel T. Nobled ; A. Denene Blackwoodd ; Julie Kinzelmane ; Tamara Anan&rsquo ; evae ; Rebecca N. Bushonf ; Erin A. Stelzerf ; Valarie J. Harwoodg ; Katrina V. Gordong ; Christopher Sinigallianoh
- 关键词：Enterococci ; qPCR ; Interference ; Performance ; EPA Method 1609 ; EPA Method 1611
- 刊名：Journal of Microbiological Methods
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：123
- 期：Complete
- 页码：114-125
- 全文大小：795 K
文摘
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Water samples from diverse U.S sites were analyzed by EPA qPCR Methods 1611 and 1609.
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Control assays indicated that matrix interference was absent in most but not all samples.
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Method 1609 was superior to Method 1611 in terms of lower control assay failure rates.
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Accepted sample analyses was evaluated by recoveries of enterococci matrix spikes.
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Acceptable recoveries were obtained in up to > 98% of analyses using ΔΔCt adjustments.