Quantitative mass spectrometry-based multiplexing compares the abundance of 5000 S. cerevisiae proteins across 10 carbon sources
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- 作者：Joao A. Paulo ; joao_paulo@hms.harvard.edu" class="auth_mail" title="E-mail the corresponding author ; Jeremy D. O'Connell ; Robert A. Everley ; Jonathon O'Brien ; Micah A. Gygi ; Steven P. Gygi ; sgygi@hms.harvard.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：TMT ; tandem mass tag
- 刊名：Journal of Proteomics
- 出版年：2016
- 出版时间：4 October 2016
- 年：2016
- 卷：148
- 期：Complete
- 页码：85-93
- 全文大小：936 K
文摘
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Over 5000 yeast proteins are quantified across 10 carbon sources in a single experiment.
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Five carbohydrate carbon sources: maltose, trehalose, fructose, sucrose, glucose.
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Five non-carbohydrate carbon sources: glycerol, acetate, pyruvate, lactic acid, oleate.
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MS3-SPS quantitative proteomics performed on an Orbitrap Fusion Lumos mass spectrometer.
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Major alterations occurred in metabolic pathways and mitochondrial proteins.