Effect of molecular weight of condensed tannins from warm-season perennial legumes on ruminal methane production in?vitro

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• 作者：Harley D. Naumann ; Luis O. Tedeschi ; James P. Muir ; Barry D. Lambert ; Merwyn M. Kothmann
• 关键词：Condensed tannin ; Greenhouse gas ; Methane ; Molecular weight ; Warm-season perennial legume
• 刊名：Biochemical Systematics and Ecology
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：50
• 期：Complete
• 页码：154-162
• 全文大小：348 K

文摘

The objectives of the present study were to determine if the molecular weight of condensed tannins (CT) from warm-season perennial legumes affects the biological activity of CT relative to suppression of methane (CH₄) production by ruminants, and to identify potential North American native forage plants to use for mitigation of enteric CH₄ emission. Eight North American native warm-season perennial legumes were evaluated: Leucaena retusa Benth. (littleleaf leadtree), Desmanthus illinoensis (Michx.) MacMill. Ex B.L. Rob. & Fernald (Illinois bundleflower), Lespedeza stuevei Nutt. (tall lespedeza), Mimosa strigillosa Torr. & A. Gray (powderpuff), Neptunia lutea (Leavenworth) Benth. (yellow puff), two ecotypes of Acacia angustissima var. hirta (Nutt.) B.L. Rob (prairie acacia), and Desmodium paniculatum (L.) DC. var. paniculatum (panicledleaf ticktrefoil). Two introduced legumes were also included: Arachis glabrata Benth. (rhizoma peanut) and Lespedeza cuneata (Dum. Cours.) G. Don (sericea lespedeza). Forages were fermented with cattle rumen fluid for 48?h anaerobically using an in?vitro gas production technique. D. paniculatum, L. stuevei, and M. strigillosa were high in CT, ranging from 11.7 to 12.5 % . D. illinoensis, L. cuneata, N. lutea, and two ecotypes of A. angustissima var. hirta had less CT (P?<?0.05), ranging from 8.1 to 8.9 % , whereas L. retusa and A. glabrata had the least CT (P?<?0.05), measuring 3.2 and 0.5 % , respectively. Weight-average molecular weight (M_W) of CT ranged from 1483?Da for L. cuneata to 552?Da for L. stuevei. In?vitro CH₄ production was greatest for L. retusa and A. glabrata at 40.7?mg/g DM and 38.2?mg/g DM, respectively. The least amount of in?vitro CH₄ was produced by fermentation of two ecotypes of A. angustissima var. hirta, which measured 0.8 and 0.6?mg/g DM, respectively. In?vitro CH₄ production regressed on CT M_W resulted in a R² of 0.0009 (P?=?0.80), strongly suggesting that CT M_W does not explain the biological activity of in?vitro CH₄ production by the forage legumes surveyed. Five of the seven North American native warm-season perennial legumes have promise for use in ruminant diets for the purpose of CH₄ emission mitigation.