A practical approach for predicting retention time shifts due to pressure and temperature gradients in ultra-high-pressure liquid chromatography

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• 作者：Dennis Å ; sberg^a ; Marcin Chutkowski^b ; Marek Leśko^b ; Jö ; rgen Samuelsson^a ; Krzysztof Kaczmarski^b ; ^{kkaczmarski@prz.edu.pl} ; Torgny Fornstedt^a ; ^{torgny.fornstedt@kau.se}
• 关键词：UHPLC ; Temperature ; Pressure effects ; Gradient effects ; Method development ; Retention time
• 刊名：Journal of Chromatography A
• 出版年：2017
• 出版时间：6 January 2017
• 年：2017
• 卷：1479
• 期：Complete
• 页码：107-120
• 全文大小：1930 K
• 卷排序：1479

文摘

Pressure and temperature gradients in UHPLC can cause selectivity shifts. An empirical, easy-to-use approach for predicting these shifts was developed. The approach requires few experiments and has straightforward calculations. Validation showed that retention times were predicted with relative errors <1%. Selectivity shifts in quality control for omeprazole was accurately predicted.