The immunostimulatory effect of hot-water extract of Gracilaria tenuistipitata on the white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus

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• 作者：Hou ; Wen-Ying ; Chen ; Jiann-Chu
• 关键词：Litopenaeus vannamei ; Vibrio alginolyticus ; Hot-water extract of Gracilaria tenuistipitata ; Total haemocyte count ; Phenoloxidase activity ; Respiratory burst ; Superoxide dismutase activity ; Phagocytic activity ; Clearance efficiency
• 刊名：Fish and Shellfish Immunology
• 出版年：2005
• 出版时间：August, 2005
• 年：2005
• 卷：19
• 期：2
• 页码：127-138
• 全文大小：324 K

文摘

The total haemocyte count (THC), phenoloxidase activity, respiratory burst, superoxide dismutase (SOD) activity, phagocytic activity and clearance efficiency to the pathogen Vibrio alginolyticus were examined in the white shrimp Litopenaeus vannamei (10.3±1.5 g) injected individually with hot-water extract of Gracilaria tenuistipitata at 4 or 6 μg g⁻¹. L. vannamei receiving hot-water extract of G. tenuistipitata at either dose increased significantly its THC, phenoloxidase activity, and respiratory burst after 2 days. L. vannamei received hot-water extract of G. tenuistipitata at 6 μg g⁻¹ increased its phagocytic activity and clearance efficiency to V. alginolyticus after 1 day. In another experiment, L. vannamei which had been injected with hot-water extract of G. tenuistipitata were challenged with V. alginolyticus at 2×10⁶ colony-forming units (cfu) shrimp⁻¹ and then placed in seawater of 34‰. The survival of shrimp that received hot-water extract of G. tenuistipitata at 6 μg g⁻¹ was significantly higher than that of shrimp that received saline and the control shrimp after 3 days, and at the termination of the experiment (6 days after the challenge). It is therefore concluded that L. vannamei receiving the hot-water extract of G. tenuistipitata at 6 μg g⁻¹ or less increased its immune ability and resistance to V. alginolyticus infection.