Dietary administration of a Gracilaria tenuistipitata extract enhances the immune response and resistance against Vibrio alginolyticus and white spot syndrome virus in the white shrimp Litopenaeus vannamei

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• 作者：Nuttarin  ; Sirirustananun^a ; Jiann-Chu  ; Chen^a ;   ; ^{jcchen@mail.ntou.edu.tw} ; Yong-Chin  ; Lin^a ; Su-Tuen  ; Yeh^a ; Chyng-Hwa  ; Liou^a ; Li-Li  ; Chen^b ; Su Sing  ; Sim^a ; Siau Li  ; Chiew^a
• 关键词：Litopenaeus vannamei ; Gracilaria tenuistipitata extract ; Dietary administration ; Vibrio alginolyticus ; White spot syndrome virus
• 刊名：Fish & Shellfish Immunology
• 出版年：2011
• 出版时间：December 2011
• 年：2011
• 卷：31
• 期：6
• 页码：848-855
• 全文大小：1049 K

文摘

The haemogram, phenoloxidase (PO) activity, respiratory bursts (RBs), superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity, lysozyme activity, and the mitotic index of haematopoietic tissue (HPT) were examined after the white shrimp Litopenaeus vannamei had been fed diets containing the hot-water extract of Gracilaria tenuistipitata at 0 (control), 0.5, 1.0, and 2.0?g?kg^? for 7?5?days. Results indicated that these parameters directly increased with the amount of extract and time, but slightly decreased after 35?days. RBs, SOD activity, and GPx activity reached the highest levels after 14?days, whereas PO and lysozyme activities reached the highest levels after 28?days. In a separate experiment, white shrimp L.?vannamei, which had been fed diets containing the extract for 14?days, were challenged with Vibrio alginolyticus at 2?¡Á?10⁶?cfu?shrimp^? and white spot syndrome virus (WSSV) at 1?¡Á?10³ copies shrimp^?, and then placed in seawater. The survival rate of shrimp fed the extract-containing diets was significantly higher than that of shrimp fed the control diet at 72?44?h post-challenge. We concluded that dietary administration of the G.?tenuistipitata extract at ?.0?g?kg^? could enhance the innate immunity within 14?days as evidenced by the increases in immune parameters and mitotic index of HPT in shrimp and their enhanced resistance against V.?alginolyticus and WSSV infections. Shrimp fed the extract-containing diets showed a higher and continuous increase in the humoral response indicating its persistent role in innate immunity.