Cytotoxicity of carteolol to human corneal epithelial cells by inducing apoptosis via triggering the Bcl-2 family protein-mediated mitochondrial pro-apoptotic pathway
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- 作者：Ming Shan ; Ting-Jun Fan ; tjfan@ouc.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：AIF ; apoptosis inducing factor ; ANOVA ; one-way analysis of variance ; AO/EB ; acridine orange/ethidium bromide ; CPE ; cytopathic effect ; Cyt. c ; cytochrome c ; DAB ; 3 ; 3&prime ; N-diaminobenzidine tertrahydrochloride ; FBS ; fetal bovine serum ; FCM ; flow cytometry ; HCEP ; human corneal epithelium ; JC-1 ; 5 ; 5&prime ; 6 ; 6&prime ; -tetrachloro-1.1&prime ; 3 ; 3&prime ; -tetraethybenzimida ; MTP ; mitochondrial transmembrane potential ; MTT ; methyl thiazolyl tetrazolium ; PI ; propidium iodide ; PM ; plasma membrane ; PMSF ; p
- 刊名：Toxicology in Vitro
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：35
- 期：Complete
- 页码：36-42
- 全文大小：1248 K
文摘
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Carteolol has cytotoxicity to human corneal epithelial cells in vitro.
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The cytotoxicity of carteolol is dose- and time-dependent.
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Carteolol induces G1-phase arrest and apoptosis of these cells.
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The carteolol-induced apoptosis is mediated by Bcl-2 family proteins.
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The pro-apoptotic pathway of carteolol is mitochondria-dependent.