The sea urchin mitochondrial transcription factor A binds and bends DNA efficiently despite its unusually short C-terminal tail
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- 作者：Christopher S. Malarkeya ; 1 ; Claudia Lionettib ; 1 ; Stefania Deceglieb ; 1 ; Marina Robertib ; Mair E.A. Churchillc ; Palmiro Cantatoreb ; d ; palmiro.cantatore@uniba.it" class="auth_mail" title="E-mail the corresponding author ; Paola Loguercio Polosab ; paolaannamaria.loguerciopolosa@uniba.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：Sea urchin mitochondria ; HMG box proteins ; Mitochondrial transcription factor A (TFAM) ; FRET ; DNA-bending
- 刊名：Mitochondrion
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：29
- 期：Complete
- 页码：1-6
- 全文大小：864 K
文摘
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TFAM from sea urchin (suTFAM) displays a very short C-terminal tail.
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suTFAM distorts DNA at a great extent and recognizes specific DNA with high affinity similarly to the human TFAM.
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Mutant versions of TFAM at the putative DNA intercalating and hydrogen bonding residues were produced.
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Those residues are responsible for the strong DNA bending and the great DNA binding affinity.