Cellular responses to TGF¦Â and TGF¦Â receptor expression in human colonic epithelial cells require CaSR expression and function

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• 作者：Navneet Singh¹ ; Guangming Liu ; Subhas Chakrabarty ; ^{schakrabarty@siumed.edu}
• 关键词：TGF¦Â ; transforming growth factor ¦Â ; CaSR ; calcium sensing receptor ; shRNA&ndash ; CaSR ; shRNA expression vector targeting the CaSR ; RIRII ; TGF¦Â receptor RIRII
• 刊名：Cell Calcium
• 出版年：2013
• 出版时间：May-June, 2013
• 年：2013
• 卷：53
• 期：5-6
• 页码：366-371
• 全文大小：1381 K

文摘

CaSR and TGF¦Â are robust promoters of differentiation in the colonic epithelium. Loss of cellular responses to TGF¦Â or loss of CaSR expression is tightly linked to malignant progression. Human colonic epithelial CBS cells, originally developed from a differentiated human colon tumor, retain CaSR expression and function, TGF¦Â responsiveness and TGF¦Â receptor expression. Thus, these cells offer a unique opportunity in determining the functional linkage (if any) between CaSR and TGF¦Â. Knocking down CaSR expression abrogated TGF¦Â-mediated cellular responses and attenuated the expression of TGF¦Â receptors. Ca²⁺ or vitamin D treatment induced CaSR expression with a concurrent up-regulation of TGF¦Â receptor expression. Ca²⁺ or vitamin D, however, did not induce CaSR in CaSR knocked down cells and without CaSR; there was no up-regulation of TGF¦Â receptor. It is concluded that TGF¦Â receptor expression and TGF¦Â mediated responses requires CaSR expression and function.