High-level soluble expression of a thermostable xylanase from thermophilic fungus Thermomyces lanuginosus in Escherichia coli via fusion with OsmY protein
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- 作者：Yilin Le ; leyilin@163.com" class="auth_mail ; Huilei Wang
- 关键词：Xylanase ; Fusion protein ; Soluble expression ; Escherichia coli ; Thermomyces lanuginosus
- 刊名：Protein Expression and Purification
- 出版年：July 2014
- 年：2014
- 卷：99
- 期：Complete
- 页码：1-5
- 全文大小：439 K
文摘
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We constructed an expression vector pET-OsmY-xynA.
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The fusion protein was expressed as soluble form without IPTG induction.
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Induction of the fusion protein is due to auto-induction.
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The fusion protein was purified and characterized.
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