Extracellular vesicles in human follicular fluid do not promote coagulation

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• 作者：Cordula Franz^a ; Anita N. B&ouml ; ing^b ; Markus Montag^c ; Thomas Strowitzki^d ; Udo R. Markert^e ; Sebastiaan Mastenbroek^f ; Rienk Nieuwland^b ; Bettina Toth^d ; ^{Bettina.toth@med.uni-heidelberg.de" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：extracellular vesicles ; exosomes ; follicular fluid ; microparticles ; ovary
• 刊名：Reproductive BioMedicine Online
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：33
• 期：5
• 页码：652-655
• 全文大小：524 K

文摘

Body fluids contain extracellular vesicles expressing tissue factor on their surface and serve as an additional trigger for coagulation. During the menstrual cycle ovarian tissue restoration is mandatory and it is unknown whether follicular fluid might provide procoagulant substances. Within an observational study, follicular fluid from women undergoing IVF/intracytoplasmic sperm injection (ICSI) was analysed by fluorescence-activated cell sorting (FACS), electron microscopy, resistive pulse sensing (RPS), nanoparticle-tracking analysis (NTA) and fibrin generation tests (FGT). The presence of extracellular vesicles, especially CD9-positive extracellular vesicles in follicular fluid, was proven. However, clotting tests revealed no procoagulant properties of the detected extracellular vesicles.