Upregulation of STREX splice variant of the large conductance Ca2+-activated potassium (BK) channel in a rat model of mesial temporal lobe epilepsy
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- 作者：Boris S. Ermolinsky ; Frank Skinner ; Ileana Garcia ; Massoud F. Arshadmansab ; Luis F. Pacheco Otalora ; Masoud M. Zarei ; Emilio R. Garrido-Sanabria
- 关键词：Kcnma1 ; ZERO ; BK channels ; Delta– ; delta cycle threshold ; MaxiK ; TaqMan ; Seizures ; Pilocarpine ; Rats
- 刊名：Neuroscience Research
- 出版年：2011
- 出版时间：January 2011
- 年：2011
- 卷：69
- 期：1
- 页码：73-80
- 全文大小：392 K
文摘
Functional properties of large conductance Ca2+ activated potassium (BK) channels are determined by complex alternative splicing of the Kcnma1 gene encoding the alpha pore-forming subunit. Inclusion of the STREX exon in a C-terminal splice site is dynamically regulated and confers enhanced Ca2+ sensitivity and channel inhibition via cAMP-dependent phosphorylation. Here, we describe a real time quantitative PCR (qPCR) approach to investigate relative changes in the expression of STREX and ZERO splice variants using a newly designed set of probes and primers for TaqMan-based qPCR analysis of cDNA from the rat dentate gyrus at different time points following pilocarpine-induced status epilepticus. Reduction in Kcnma1 gene expression is associated with a relative increase of STREX splice variant. Relative expression of STREX variant mRNA was increased at 10 days and at more than 1 month following status epilepticus. The biological consequences of seizure-related changes in alternative splicing of Kcnma1 deserve additional investigation.