mRNA expression profile of the TNF-α system in LH-induced bovine preovulatory follicles and effects of TNF-α on gene expression, ultrastructure and expansion of cumulus-oocyte complexes cultured in vitro

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• 作者：A.W.B. Silva^a ; F.T.G. Bezerra^a ; W.G. Glanzner^b ; J.T. dos Santos^b ; A.M.P. Dau^b ; M.T. Rovani^b ; G.F. Ilha^b ; J.J.N. Costa^a ; E.V. Cunha^a ; M.A.M. Donato^d ; C.A. Peixoto^d ; P.B.D. Gonç ; alves^b ; V. Bordignon^c ; J.R.V. Silva^a ; ^{jrvsilva@ufc.br}
• 关键词：Ovary ; Ovulation ; Cell culture ; Cytokine ; Protein ; Cattle
• 刊名：Theriogenology
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：90
• 期：Complete
• 页码：1-10
• 全文大小：2092 K
• 卷排序：90

文摘

The proteins by TNF-α and its receptors (TNFR1 and TNFR2), are present in antral follicles compartments. GnRH treatment increases TNF-α mRNA level in granulosa cells of preovulatory follicles in vivo. In vitro, expression of TNF-α mRNA in cumulus cells is also upregulated by gonadotropins. TNF-α maintains ultrastructure and regulates gene expression during COC culture.