Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp. PCC 6803
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- 作者：Ancy Joseph ; Shimpei Aikawa ; Kengo Sasaki ; Hiroshi Teramura ; Tomohisa Hasunuma ; Fumio Matsuda ; Takashi Osanai ; Masami Yokota Hirai ; Akihiko Kondo
- 关键词：GS ; glutamine synthetase ; GOGAT ; glutamate synthase ; HPLC ; high performance liquid chromatography ; LC/QqQ-MS ; liquid chromatography/triple quadrupole mass spectrometry ; OD ; optical density ; TCA ; tricarboxylic acid ; 2-OG ; 2-oxoglutarate
- 刊名：FEBS Letters
- 出版年：31 January, 2014
- 年：2014
- 卷：588
- 期：3
- 页码：466-471
- 全文大小：971 K
文摘
Rre37 (sll1330) in a cyanobacterium Synechocystis sp. PCC 6803 acts as a regulatory protein for sugar catabolic genes during nitrogen starvation. Low glycogen accumulation in 螖rre37 was due to low expression of glycogen anabolic genes. In addition to low 2-oxoglutarate accumulation, normal upregulated expression of genes encoding glutamate synthases (gltD and gltB) as well as accumulation of metabolites in glycolysis (fructose-6-phosphate, fructose-1,6-bisphosphate, and glyceraldehyde-3-phosphate) and tricarboxylic acid (TCA) cycle (oxaloacetate, fumarate, succinate, and aconitate) were abolished by rre37 knockout. Rre37 regulates 2-oxoglutarate accumulation, glycogen accumulation through expression of glycogen anabolic genes, and TCA cycle metabolites accumulation.