Cytoplasmic polyhedrosis virus-induced differential gene expression in two silkworm strains of different susceptibility

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• 作者：Kun Gao^a ; ^b ; ^c ; Xiang-yuan Deng^a ; He-ying Qian^b ; ^c ; Guang-xing Qin^b ; ^c ; Cheng-xiang Hou^b ; ^c ; Xi-jie Guo^b ; ^c ; ^{guoxijie@126.com" class="auth_mail}
• 关键词：DGE ; digital gene expression ; KEGG ; Kyoto Encyclopedia of Genes and Genomes ; VMPs ; vitelline membrane proteins ; qRT-PCR ; quantitative real-time PCR method ; SilkDB ; silkworm genome database ; TPM ; transcripts per million clean tags ; FDR ; false discovery rate ; GO ; gene ontology ; BmCPV ; Bombyx mori cytoplasmic polyhedrosis virus ; IBP2 ; insulin-related peptide-binding protein 2 ; IGFBP7 ; insulin-like growth factor-binding protein ; p37k ; 37  ; kDa protease precursor ; BHV-1 ; bovine herpes virus 1 ; CC2
• 刊名：Gene
• 出版年：15 April, 2014
• 年：2014
• 卷：539
• 期：2
• 页码：230-237
• 全文大小：1217 K

文摘

Digital gene expression (DGE) was performed to investigate the gene expression profiles of 4008 and p50 silkworm strains at 48 h after oral infection with BmCPV. 3,668,437 clean tags were identified in the BmCPV-infected p50 silkworms and 3,540,790 clean tags in the control p50. By contrast, 4,498,263 clean tags were identified in the BmCPV-infected 4008 silkworms and 4,164,250 clean tags in the control 4008. A total of 691 differentially expressed genes were detected in the infected 4008 DGE library and 185 were detected in the infected p50 DGE library, respectively. The expression profiles identified some important differentially expressed genes involved in signal transduction, enzyme activity and apoptotic changes, some of which were verified using quantitative real-time PCR (qRT-PCR). These results provide important clues on the molecular mechanism of BmCPV invasion and resistance mechanism of silkworms against BmCPV infection.