Overcoming the membrane barrier: Recruitment of γ-glutamyl transferase for intracellular release of metabolic cargo from peptide vectors

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• 关键词：Retrobiosynthesis ; Portage transport ; Gamma-glutamyl transferase ; Peptide transport ; Xenobiotic import
• 刊名：Metabolic Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：60-70
• 全文大小：1237 K
• 卷排序：39

文摘

A synthetic transport system to overcome the membrane barrier of E. coli. Intracellular release of cargo molecules using γ-glutamyl transferase (GGT). Expression of GGT in the cytoplasm of E. coli leads to toxic effects. Toxic effects can be mitigated through ribosome binding site engineering.