Beyond just being foot soldiers - osmotin like protein (OLP) and chitinase (Chi11) genes act as sentinels to confront salt, drought, and fungal stress tolerance in tomato
详细信息 查看全文
- 作者：S. Anil Kumara ; P. Hima Kumaria ; G. Jawahara ; S. Prashantha ; Prashanth Suravajhalab ; Ramesh Katamc ; Pramod Sivand ; K.S. Raod ; P.B. Kirtie ; P.B. Kavi Kishora ; pbkavi@yahoo.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Abiotic stress ; Biotic stress ; Chitinase ; Co-Immunoprecipitation ; Osmotin like protein ; Pathogenesis-related proteins ; Phosphofructokinase2
- 刊名：Environmental and Experimental Botany
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：132
- 期：Complete
- 页码：53-65
- 全文大小：4798 K
文摘
- •
Transgenic tomato plants were developed for tolerance to both biotic and abiotic stresses by heterologous expression of osmotin like protein (OLP) and chitinase (Chi11) genes.
- •
Transgenics showed enhanced tolerance to salt, drought, and fungal stresses compared to untransformed controls. Co-Immunoprecipitation revealed that Chi11 co-expresses with phosphofructokinase2 (PFK2), an important enzyme in glycolysis.
- •
Quantitative real-time PCR results revealed higher expression of OLP, Chi11, and PFK2 under both biotic and abiotic stresses in transgenics.
- •
Our findings enhance the use of multiple genes to confer stress tolerance. This work unveils a new molecular player PFK2, which may play a role in enhanced root biomass under stress.