Emodin ameliorates high-fat-diet induced insulin resistance in rats by reducing lipid accumulation in skeletal muscle

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• 作者：Yanni Cao^a ; ¹ ; Shufang Chang^b ; ¹ ; Jie Dong^a ; Shenyin Zhu^a ; ^{zhushenyin0486@sina.com" class="auth_mail" title="E-mail the corresponding author} ; Xiaoying Zheng^a ; Juan Li^a ; Rui Long^a ; Yuanda Zhou^a ; Jianyu Cui^a ; Ye Zhang^a
• 关键词：ANOVA ; one-way analysis of variance ; ELISA ; enzyme-linked immunosorbent assay ; FATP1 ; fatty acid transport protein 1 ; FATP4 ; fatty acid transport protein 4 ; FAT/CD36 ; transporter fatty acid translocase ; FABPpm ; plasma membrane-associated fatty acid protein ; FBG ; fasting blood glucose ; GIR ; glucose infusion rate ; HFD ; high fat diet ; HOMA2_IR ; homeostasis model assessment insulin resistance index ; LCFA ; long-chain fatty acid ; LDL-C ; low density lipoprotein cholesterol ; NFD ; normal food diet ; qRT-P
• 刊名：European Journal of Pharmacology
• 出版年：2016
• 出版时间：5 June 2016
• 年：2016
• 卷：780
• 期：Complete
• 页码：194-201
• 全文大小：2411 K

文摘

Emodin, an anthraquinone derivative isolated from root and rhizome of Rheum palmatum, has been reported to have promising anti-diabetic activity. The present study was to explore the possible mechanism of emodin to ameliorate insulin resistance. Insulin resistance was induced by feeding a high fat diet to Sprague-Dawley rats. The blood glucose and lipid profiles in serum were measured by an enzymatic method, and a hyperinsulinaemic-euglycaemic clamp was used to evaluate insulin resistance. L6 cells were cultured and treated with palmitic acid and emodin. The lipid content was assayed in the soleus muscle and L6 cells by Oil Red O staining. Western blot, qRT-PCR, and immunohistochemical staining were used to detect the following in the rat soleus muscle and L6 cells: protein levels, mRNA levels of FATP1, FATP4, transporter fatty acid translocase (FAT/CD36), and plasma membrane-associated fatty acid protein (FABPpm). We found that blood glucose, triglyceride (TG), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) were significantly decreased in the emodin group. Oil Red O staining and the level of TG in skeletal muscle and L6 cells confirmed that lipid deposition decreased after treatment with emodin. Furthermore, the protein levels and mRNA levels of FATP1 in skeletal muscle and in L6 cells of rats were significantly decreased, yet the protein levels and mRNA levels of FATP4, FAT/CD36 and FABPpm did not drop off significantly. The study suggest that emodin ameliorates insulin resistance by reducing FATP1-mediated skeletal muscle lipid accumulation in rats fed a high fat diet.