Antidiabetic effect of total saponins from Polygonatum kingianum in streptozotocin-induced daibetic rats

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• 作者：Jian-mei Lu^a ; ^b ; Yan-fang Wang^a ; ^b ; Hong-li Yan^a ; ^b ; Pei Lin^a ; ^b ; Wen Gu^a ; ^b ; Jie Yu^a ; ^b ; ^{cz.yujie@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：AMPK ; Adenosine monophosphate activated protein kinase ; DM ; Diabetes mellitus ; FBG ; Fasting blood glucose ; FBP ; Fructose 6 phosphate kinase2 ; FINS ; Fasting insulin ; GK ; Glucose kinase ; GLUT4 ; Glucose transporter subtype-4 ; G6P ; Glucose-6-phosphatase ; HFD ; High-fat diet ; HSL ; Hormone sensitive lipase ; LOD ; Limit of detection ; LOQ ; Limit of quantitation ; MET ; Metformin hydrochloride ; PEPCK ; Phosphoenolpyruvate carboxykinase ; PHK ; Phosphorylase kinase ; PKA ; Protein kinase ; PPAR-γ ; Peroxysome prolif
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：17 February 2016
• 年：2016
• 卷：179
• 期：Complete
• 页码：291-300
• 全文大小：2245 K

文摘

Polygonatum kingianum has been used in the prevention and treatment of diabetes, hyperlipidemia and related metabolic syndrome in Asian counties for centuries. In this study, the blood glucose regulation activity and mechanism of total saponins from P. kingianum (TSPK) were investigated in streptozotocin (STZ)-induced diabetic rats in this research.

Methods

60">TSPK (0.025 g/kg and 0.1 mg/kg) was administrated by gavage to STZ-induced diabetic rats for 8 weeks. Changes of body weight, food intakes, blood glucose, serum insulin and lipid indexes were observed. Genome-wide expression profiling was applied to explore the gene expression alternation after treated with TSPK. Expressions of adenosine monophosphate activated protein kinase (AMPK), phosphoenolpyruvate carboxykinase (PEPCK), the relative transcript level of glucose kinase and glucose-6-phosphatase (GK/G6P) in the liver were investigated. Meanwhile, contents of AMPK, and glucose transporter subtype-4 (GLUT4) in skeletal muscle, and peroxysome proliferator-activated receptor (PPAR-γ) in adipose tissue were investigated.

Results

65">TSPK could effectively alleviate hyperglycemia and hyperlipidemia in diabetic rats. Genome-wide expression profiling showed that TSPK up-regulated the expression of GLUT4 while down-regulated the expression of G6P in insulin signal pathway. In the liver, the expression of AMPK and GK are increased. Further more, TSPK promoted the expressions of GLUT4 in skeletal muscle, and PPAR-γ in adipose tissue, respectively.

Conclusion

These results provide possible mechanisms for the antidiabetic effects of TSPK. TSPK could promote not only glycogenesis but also glucose utilization in peripheral tissue. Our results suggested that TSPK may be used as adjuvant therapy to control blood glucose and insulin resistance in type 2 diabetic individuals.