Bio-functional surfaces for the immunocapture of AGO2-bound microRNAs
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- 作者：V. Vaghia ; C. Potricha ; b ; cpotrich@fbk.eu" class="auth_mail" title="E-mail the corresponding author ; L. Lunellia ; b ; P. Faccic ; L. Pasquardinia ; 1 ; L. Vanzettid ; C. Pederzollia
- 关键词：GPTMS ; 3-glycidyloxypropyl-trimethoxysilane ; APTES ; (3-aminopropyl)-triethoxy-silane ; PEG-silane ; (2-[methoxy-(polyethyleneoxy)propyl]trimethoxysilane) 21&ndash ; 24 ; AP ; 0.1% APTES ; + ; 0.9% PEG-silane surface ; TG-SO ; thermally grown silicon oxide ; AGO2 ; Argonaute 2 protein ; XPS ; X-ray photoelectron spectroscopy ; AFM ; atomic force microscopy ; QCM ; quartz crystal microbalance
- 刊名：Colloids and Surfaces B: Biointerfaces
- 出版年：2016
- 出版时间：1 October 2016
- 年：2016
- 卷：146
- 期：Complete
- 页码：746-753
- 全文大小：1213 K
文摘
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A new biosurface for the purification of microRNAs in protein complexes was set up.
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The biosurface was tested for miRNA purification from cell lysates and human plasma.
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Different amounts of miRNAs were found in the different biological samples tested.
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The different amounts are in agreement with the physiological miRNA level.