Preventive and curative effect of Pistacia lentiscus oil in experimental colitis

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• 作者：Mustafa S. Naouar^a ; Lilia Zouiten Mekki^a ; ^b ; ^{w.mekki@laposte.net" class="auth_mail" title="E-mail the corresponding author} ; Lamia Charfi^c ; Jalel Boubaker^b ; Azza Filali^b
• 关键词：Inflammatory bowel disease ; 2 ; 4 ; 6-trinitrobenzene sulfonic acid-induced colitis ; Wistar rats ; Lentisc oil ; Fatty acid
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：83
• 期：Complete
• 页码：577-583
• 全文大小：2233 K

文摘

to investigate the anti-inflammatory effect of the Pistacia lentiscus oil in experimental colitis model.

Materials and methods

Colitis was induced in male rats by instillation of 2,4,6-trinitrobenzenesulfonic acid (TNBS) in all groups. The experimental groups consisted of: 5 rats received Lentisc oil 2months before colitis induction (preventive group), 5 rats received the oil on the day of colitis induction (curative group) and 5 control rats. Lentisc oil was extracted from the ripe fruit of the plant by the cold press method and was analyzed by spectro-chromatography. Lentisc oil has been inserted with a standard diet at the dose of 30 mg oil/100 g of food/rat.

Results

The lentisc oil sample is composed mainly by Oleic acid (47.96%), Palmitic acid (27.94%) and Linoleic acid (20.22%).There was a significant difference between control rats and treated rats with lentisc oil concerned body mass (p = 0.009), bleeding index (p = 0.005 and p = 0.018) and diarrhea (p = 0.012). Histological examination revealed a clear difference between the control and preventive groups with disappearance of erosion, decreased of cryptitis, irregular crypts and crypt loss in the preventive group. Curative group showed a significant decrease of ulceration, hyperplasia, cryptitis, irregular crypts and crypt loss compared to the control group. There was an attenuation of inflammation in the preventive group compared to the curative group without statistically significant.

Conclusion

Lentisc oil administration could provide a protective effect on intestinal inflammation in colitis rats induced by TNBS mainly when it is administered at a young age in preventive mode. This beneficial effect would involve a modification of arachidonic acid metabolism.