Neuroprotective effect of Spilanthes acmella Murr. on pesticide-induced neuronal cells death

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• 作者：Wilasinee Suwanjang¹ ; Bongkot Khongniam¹ ; Sujittra Srisung² ; Supaluk Prachayasittikul³ ; Virapong Prachayasittikul⁴ ; ^{virapong.pra@mahidol.ac.th}
• 关键词：Spilanthes acmella Murr. ; Pirimicarb ; Pesticide ; Neuronal cell ; Calpain ; Calpastatin
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：10
• 期：1
• 页码：35-41
• 全文大小：1262 K
• 卷排序：10

文摘

To investigate protective effects of Spilanthes acmella (S. acmella) Murr. extracts against pesticide-induced neuronal cells death and to elucidate the underlying molecular mechanism in dopaminergic (SH-SY5Y) cells lines.MethodsCell viability of SH-SY5Y cells was studied by treating the cells with various concentration of pirimicarb for 24 h. Neuroprotective effect of S. acmella Murr. extracts was investigated by adding the plant extracts to the medium for 24 h prior to the incubation with 100 μM H2O2 or with pirimicarb for 24 h. Control-untreated cells were incubated with the culture medium. Cell viability was measured by MTT assay, calpain and calpastatin expressions were analyzed by Western blotting and immunocytochemistry.ResultsPretreatment of SH-SY5Y cells with S. acmella Murr. extracts (1 μg/mL) for 24 h significantly increased the dopaminergic neurons in pirimicarb-induced neurotoxicity. In addition, pretreatment with the S. acmella Murr. extracts led to decreased calpain but increased calpastatin protein levels.ConclusionS. acmella Murr. extracts exerted neuroprotective effect, via an alteration of calcium homeostasis, against pirimicarb induced neurotoxicity. The S. acmella Murr. might be a potential natural candidate with neuroprotective activity.