Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) in Latin-American migrants in Barcelona (Spain)

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• 作者：Alba Abras^a ; ^b ; ^c ; ¹ ; Montserrat Gá ; llego^a ; ^b ; ¹ ; ^{mgallego@ub.edu} ; Carmen Muñ ; oz^d ; ^e ; ^f ; ¹ ; Natalia A Juiz^g ; Juan Carlos Ramí ; rez^g ; Carolina I Cura^g ; Silvia Tebar^a ; ^b ; Anna Ferná ; ndez-Aré ; valo^a ; ^b ; ^e ; Marí ; a-Jesú ; s Pinazo^b ; Leonardo de la Torre^b ; Elizabeth Posada^b ; Ferran Navarro^d ; ^e ; ^f ; Paula Espinal^e ; Cristina Ballart^a ; ^b ; Montserrat Portú ; s^a ; Joaquim Gascó ; n^b ; Alejandro G Schijman^g ; ¹
• 关键词：Chagas disease ; Trypanosoma cruzi ; Discrete Typing Unit ; Migration ; Real-time PCR ; Sequencing
• 刊名：Parasitology International
• 出版年：2017
• 出版时间：April 2017
• 年：2017
• 卷：66
• 期：2
• 页码：83-88
• 全文大小：422 K
• 卷排序：66

文摘

PCR flowcharts and satellite-DNA sequencing were used to genotype Trypanosoma cruzi. Parasitic load of chronic patients was limiting for flowcharts but not for satellite-DNA sequencing. Most DTUs identified belonged to samples from neonates and pediatric cases. The most identified DTU was TcV, common in Bolivian domestic cycles. A mixed infection TcV–TcII was detected for the first time simultaneously in Bolivian migrants.