Cadmium induces apoptosis differentially on immune system cell lines

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• 作者：Tsangaris ; George Th. ; Tzortzatou-Stathopoulou ; Fotini
• 关键词：Apoptosis ; Cadmium ; DNA fragmentation ; Heavy metals ; Immune system ; Immunotoxicity
• 刊名：Toxicology
• 出版年：1998
• 出版时间：July 3, 1998
• 年：1998
• 卷：128
• 期：2
• 页码：143-150
• 全文大小：1.40 M

文摘

We investigate the role of cadmium-induced apoptosis in the immune system, studying the apoptotic effect of Cd²⁺ in three human cell lines, the T-cell line CCRF-CEM, the B-cell line Raji and the lymphoblastoid cell line Molt-3. Cd²⁺ was found to be dose-dependently toxic for these cell lines, after 18 h incubation. The 50 % lethal dose (LD₅₀) for CCRF-CEM was 25±20 μM, for Molt-3 was 22.5±2.4 μM, and for Raji was 13.5±2.2 μM. DNA electrophoresis and quantitation of apoptosis after 18 h incubation with different Cd²⁺ concentrations was carried out. In CCRF-CEM cells, apoptosis was detected at 10 μM, reaching a maximum at 30 μM. In Molt-3, apoptosis was detected at 10 μM, increased thereafter and a plateau effect was observed from 30 to 50 μM Cd²⁺. In Raji, apoptosis was detected at 5 μM, while a plateau effect was observed from 20 to 30 μM Cd²⁺. The above results indicated that Raji cells were more sensitive to cadmium compared to both CCRF-CEM and Molt-3 cells, suggesting a differential Cd²⁺-induced apoptotic effect, which may disturb the immune system normal growth and development.