UPLC–MS/MS analysis of human liver cytosolic LCA sulfation.
The matrix (human liver cytosol) did not interfere with the assay.
The assay was optimized for Mg, DTT, 2-mercaptoethanol, and PAPS concentrations.
The assay was linear with respect to amount of enzyme and incubation time.
Faster and more sensitive than existing LC–MS or radiometric methods.