Ultrasensitive electrochemical detection of nucleic acid by coupling an autonomous cascade target replication and enzyme/gold nanoparticle-based post-amplification
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- 作者：Shufeng Liua ; sliu@qust.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Wenji Weia ; Yanqun Wanga ; Li Fanga ; Li Wanga ; wangli@qust.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Feng Lib ; lifeng@qust.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：DNA biosensor ; Electrochemistry ; Target replication ; signal amplification ; Gold nanoparticle
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 June 2016
- 年：2016
- 卷：80
- 期：Complete
- 页码：208-214
- 全文大小：1425 K
文摘
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An isothermal and ultrasensitive electrochemical DNA biosensor was developed.
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The coupled autonomous target replication with enzyme/gold nanoparticle-based post-amplification was proposed.
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A superior low detection limit of 0.065 fM toward target DNA could be achieved.
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It creates an opportunity for the effective coupling of the target replication and post-amplification strategies.