Upconversion nanoparticles based FRET aptasensor for rapid and ultrasenstive bacteria detection

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• 作者：Birui Jin^a ; ^c ; Shurui Wang^b ; ^c ; Min Lin^b ; ^c ; ^{minlin@mail.xjtu.edu.cn} ; Ying Jin^d ; Shujing Zhang^d ; Xingye Cui^b ; ^c ; Yan Gong^b ; ^c ; Ang Li^f ; Feng Xu^b ; ^c ; Tian Jian Lu^c ; ^e
• 关键词：Upconversion nanoparticle ; Gold nanoparticle ; Aptamer ; E. coli ATCC 8739 ; Microorganism detection
• 刊名：Biosensors and Bioelectronics
• 出版年：2017
• 出版时间：15 April 2017
• 年：2017
• 卷：90
• 期：Complete
• 页码：525-533
• 全文大小：977 K
• 卷排序：90

文摘

A novel UCNPs based FRET aptasensor was first developed to detect bacteria. The system avoids target separation procedures and enables rapid, ultrasensitive and specific bacteria detection. The sensitivity of the developed aptasensor is comparable or even higher than that achieved in previous studies. The aptasensor was further used to detect E. coli in tap/pond water and milk.