Simultaneous quantification method for comparative pharmacokinetics studies of two major metabolites from geniposide and genipin by online mircrodialysis-UPLC-MS/MS

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• 作者：Xueju Zhang^a ; ^b ; Shu Liu^a ; Zifeng Pi^a ; ^{mslab21@ciac.ac.cn} ; Zhiqiang Liu^a ; Fengrui Song^a ; ^{songfr@ciac.ac.cn}
• 关键词：Metabolites ; Comparative pharmacokinetics ; Diabetic rat model ; Online mircrodialysis ; Mass spectrometry
• 刊名：Journal of Chromatography B
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：1041-1042
• 期：Complete
• 页码：11-18
• 全文大小：1531 K
• 卷排序：1041

文摘

A breakthrough of the simultaneous quantification of major metabolites from herb medicines was achieved without standard compounds using online mircrodialysis-ultra performance liquid chromatography-mass spectrometry. It is the first time to investigate the compare pharmacokinetics of two major metabolites, namely, genipin-1-o-glucuronic acid and genipin-monosulfate from geniposide and genipin between healthy and diabetic rats by online MD-UPLC–MS/MS. The results showed that their pharmacokinetic behaviors in type 2 diabetic rats significantly differ from healthy rats (p < 0.05). The approach could predict druggability of bioactive metabolites from their prototypes.