Persistence and the state of bovine and porcine adenoviral vector genomes in human and nonhuman cell lines

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• 作者：Anurag  ; Sharma ; Dinesh S.  ; Bangari¹ ; Sai V.  ; Vemula ; Suresh K.  ; Mittal  ; ;   ; ^{mittal@purdue.edu}
• 关键词：Adenovirus persistence ; Bovine adenovirus ; Gene therapy ; Nonhuman adenovirus ; Porcine adenovirus ; Vector safety
• 刊名：Virus Research
• 出版年：2011
• 出版时间：November 2011
• 年：2011
• 卷：161
• 期：2
• 页码：181-187
• 全文大小：881 K

文摘

The state of vector genome in transduced cells influences the duration of transgene expression and can be a safety concern if it gets integrated randomly into the host genome. Although human adenovirus (Ad) serotype 5 (HAd5) mainly persists in a linear episomal form, information regarding the state of bovine Ad serotype 3 (BAd3) and porcine Ad serotype 3 (PAd3) vector genomes in human and nonhuman cells is currently unknown. To address this issue, MDA-MB-231 (human), MDBK (bovine), PK-15 (porcine), MT1A2 (mouse) and NIH-3T3 (mouse) cell lines were infected with replication-defective BAd3, PAd3 or HAd5 vectors carrying the green fluorescent protein (GFP) gene. The persistence and the state of vector genome were assessed by quantitative real-time PCR and Southern blot hybridization, respectively. Levels of transgene and Ad gene expressions were quantified using real-time RT-PCR. Persistence of BAd3 or PAd3 vectors was comparable to that of HAd5 vector. Only the linear episomal form of the vector genome was observed with each vector. In addition, expression levels of transgene as well as viral genes by all three vectors were comparable and correlated with their transduction levels in each cell type. These results indicate comparable biologic behavior of BAd3, PAd3 and HAd5 vectors in cell culture.