Optimization and application of propidium monoazide-quantitative PCR method for viable bacterial bioaerosols

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• 作者：Ching-Wen Chang^a ; ^b ; ^c ; ^{chingwenchang@ntu.edu.tw} ; Nien-Tzu Hung^d ; Nai-Tzu Chen^a
• 关键词：viable bacteria ; quantitative PCR ; propidium monoazide ; ethidium monoazide
• 刊名：Journal of Aerosol Science
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：104
• 期：Complete
• 页码：90-99
• 全文大小：688 K
• 卷排序：104

文摘

Quantitative PCR with PMA was optimized for viable bacterial aerosols qPCR with 1.5 μg/mL of PMA showed promise for bacterial quantification Applicability of PMA-qPCR to field aerobiology was demonstrated Bacterial viability and culturability in studied environments were determined Viable but not culturable bacteria were abundantly present in field air