Knockdown of heat shock protein 70 (Hsp70) by RNAi reduces the tolerance of Artemia franciscana nauplii to heat and bacterial infection

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• 作者：Mat Taib Mimi Iryani^a ; Thomas H. MacRae^b ; ^{tmacrae@dal.ca} ; Sheethal Panchakshari^b ; Jiabo Tan^b ; Peter Bossier^c ; Mohd Effendy Abd. Wahid^a ; ^d ; Yeong Yik Sung^a ; ^d ; ^{yeong@umt.edu.my}
• 关键词：Hsp70 ; Artemia franciscana ; Nauplii ; RNA interference (RNAi) ; Thermotolerance ; Vibrio campbellii
• 刊名：Journal of Experimental Marine Biology and Ecology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：487
• 期：Complete
• 页码：106-112
• 全文大小：2153 K
• 卷排序：487

文摘

RNA interference (RNAi) was used to assess the role of the molecular chaperone Hsp70 in protecting nauplii of the crustacean, Artemia franciscana, against abiotic and biotic stressors. As revealed respectively by RT-PCR and immunoprobing of Western blots, the injection of A. franciscana females with Hsp70 dsRNA eliminated Hsp70 mRNA and protein in nauplii released from these animals. The loss of Hsp70 had no apparent effect on either embryo development or the viability of A. franciscana nauplii. The survival of nauplii lacking Hsp70, as compared to those containing the Hsp, was reduced 41% by heat stress and 34% upon Vibrio campbellii infection. The results suggest that Hsp70 assisted in maintaining protein homeostasis within stressed cells while potentially stimulating the innate immune system of A. franciscana.