Antibodies to the CP24 protein of Brucella melitensis lack diagnostic usefulness in ovine brucellosis

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• 作者：Delpino ; M. Victoria ; Cassataro ; Juliana ; Fossati ; Carlos A. ; Baldi ; Pablo C.
• 关键词：Brucellosis ; Sheep ; Diagnosis ; CP24 protein ; Ribosome recycling factor ; ELISA
• 刊名：Veterinary Microbiology
• 出版年：2003
• 出版时间：May 19, 2003
• 年：2003
• 卷：93
• 期：2
• 页码：101-107
• 全文大小：102 K

文摘

The potential diagnostic usefulness of antibodies to the ribosome recycling factor of Brucella melitensis (CP24) was assessed in sheep by an indirect ELISA with purified recombinant CP24. Sera from uninfected animals from the UK (n=44) and from local flocks (n=42), from sheep naturally infected with B. melitensis (n=12) or B. ovis (n=12), and from lambs (n=7) or pregnant ewes (n=6) vaccinated with B. melitensis Rev-1, were assayed. High specific optical densities (OD_withantigen − OD_{withoutantigen}) were obtained with both the groups of normal sera, which resulted in high cut-off values (1.414 and 1.267, respectively). Only two infected sheep yielded specific OD higher than these cut-off values. No significant difference was found between mean specific OD from B. melitensis- or B. ovis-infected sheep (0.574 and 0.472, respectively), those from vaccinated animals (0.396 and 0.400 for pregnant ewes and lambs, respectively), and those from Brucella-free animals. An inhibition ELISA with soluble CP24 confirmed the specificity of the antibodies detected in normal sera by the indirect ELISA; these antibodies belonged to the IgG class as revealed by the use of a specific conjugate. Sera from infected sheep were all positive for antibodies against lipopolysaccharides and lumazine synthase from Brucella. These results show that anti-CP24 antibodies have no diagnostic role in ovine brucellosis.