Probing mucin interaction behavior of magnetic nanoparticles

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• 作者：Vijayakumar N. Boya^a ; ^b ; Renn Lovett^a ; Saini Setua^a ; Vaibhav Gandhi^a ; Prashanth K.B. Nagesh^a ; Sheema Khan^a ; Meena Jaggi^a ; Murali M. Yallapu^a ; ^{myallapu@uthsc.edu} ; Subhash C. Chauhan^a ; ^{schauhan1@uthsc.edu}
• 关键词：Magnetic nanoparticles ; Drug delivery ; Mucoadhesive pharmaceuticals ; Mucin ; Cancer
• 刊名：Journal of Colloid and Interface Science
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：488
• 期：Complete
• 页码：258-268
• 全文大小：2945 K
• 卷排序：488

文摘

In this study, we developed iron oxide based magnetic nanoparticles (MNPs) by precipitation of iron salts in the presence of ammonia and created four different formulations: without functionality (plain MNPs, no coating), with β-cyclodextrin (MNPs+β-CD) or pluronic 127 polymer (MNPs+F-127), and both β-cyclodextrin and pluronic 127 polymer (MNPs+β-CD-F-127) functionality for its efficient use in mucosal delivery. We studied the interaction and/or binding behavior of these MNPs formulations with porcine stomach mucin using steady-state fluorescence spectroscopy, and then quantified the bound mucin from absorption studies. Toxicity of these MNPs against cervical cancer cells and red blood cells was evaluated. Ex-vivo studies were performed using freshly collected gastrointestinal, ovarian, pancreas and colon organ tissues of pig to evaluate binding and uptake phenomenon of MNPs. Transport studies of these MNPs in mucin was evaluated using Boyden’s chamber assay. All these studies together suggest that the MNPs+β-CD-F-127 formulation was strongly interacted with mucin and interestingly transported through mucin compared to other MNPs formulations. Hence, MNPs+β-CD-F-127 formulation could be a good candidate for the mucoadhesive biopharmaceuticals and drug delivery system.