Electrochemical magnetoassay coupled to PCR as a quantitative approach to detect the soybean transgenic event GTS40-3-2 in foods

详细信息    查看全文

• 作者：C. Lorena Manzanares-Palenzuela^a ; ^b ; ^cAuthor Vitae ; Isabel Mafra^a ; ^{isabel.mafra@ff.up.pt" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Joana Costa^aAuthor Vitae ; M. Fá ; tima Barroso^bAuthor Vitae ; Noemí ; de-los-Santos-Á ; lvarez^dAuthor Vitae ; Cristina Delerue-Matos^bAuthor Vitae ; M. Beatriz P.P. Oliveira^aAuthor Vitae ; M. Jesus Lobo-Castañ ; ó ; n^dAuthor Vitae ; Beatriz Ló ; pez-Ruiz^c ; ^{bealopru@quim.ucm.es" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：GMO ; Roundup Ready soybean ; Electrochemical DNA detection ; PCR ; Biosensor
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：222
• 期：Complete
• 页码：1050-1057
• 全文大小：1378 K

文摘

Simple, cost-effective and reliable tools for the quantification of genetically modified organisms (GMO) in food and feed are highly demanded to enforce labelling legislation in the EU. Herein, we report a novel method for quantitative analysis of genetically modified soybean with the event GTS-40-3-2, also known as Roundup Ready (RR) soybean, using magnetoassays with electrochemical detection, coupled to DNA amplification by end-point polymerase chain reaction (PCR). For the proposed work, two DNA sequences were targeted via hybridisation onto magnetic beads, one specific for the transgenic event and the other for the taxon or species-specific lectin gene. Enzymatic labelling was performed to obtain an electrochemically active product measured by chronoamperometry. By optimising the number of PCR cycles, among other parameters, two magnetoassays coupled to PCR were successfully accomplished and linearity was obtained in the ranges of 53–4425 and 1093–88,496 DNA copies for the event-specific and lectin sequences, respectively. The proposed method provides accurate and precise RR soybean quantitative results, being effectively compared to those obtained by real-time PCR, as the reference method. These findings confirm the suitability of the method as an alternative tool for GMO quantification.