Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris

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• 作者：Hermann Ehrlich ; Oksana V. Kaluzhnaya ; Eike Brunner ; Mikhail V. Tsurkan ; Alex ; er Ereskovsky ; Micha Ilan ; Konstantin R. Tabachnick ; Vasilii V. Bazhenov ; Silvia Paasch ; Martin Kammer ; Ren¨¦ Born ; Allison Stelling ; Roberta Galli ; Sergei Belikov ; Olga V. Petrova ; Victor V. Sivkov ; Denis Vyalikh ; Sebastian Hunoldt ; Gert W?rheide
• 关键词：Sponges ; Skeleton ; Chitin ; Chitin synthase ; Bioanalytics
• 刊名：Journal of Structural Biology
• 出版年：2013
• 出版时间：September, 2013
• 年：2013
• 卷：183
• 期：3
• 页码：474-483
• 全文大小：2831 K

文摘

This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques (¹³C solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining); we show that this sponge chitin is much closer to ¦Á-chitin, known to be present in other animals, than to ¦Â-chitin. Genetic analysis confirmed the presence of chitin synthases, which are described for the first time in a sponge. The presence of chitin in both marine (demosponges and hexactinellids) and freshwater sponges indicates that this important structural biopolymer was already present in their common ancestor.