Walker 256/B malignant breast cancer cells disrupt osteoclast cytomorphometry and activity in rats: Modulation by 伪-tocopherol acetate

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• 作者：Riadh Badraoui ; Hmed Ben-Nasr ; Selma Amamou ; Mich猫le V茅ronique El-May ; Tarek Rebai
• 关键词：VE ; vitamin E ; VES ; VE supplementation ; OC ; osteoclast ; BW ; body weight ; BV/TV ; trabecular bone volume ; Tb.Th ; trabecular thickness ; Tb.N ; trabecular number ; Tb.Sp ; trabecular separation ; OV/BV ; osteoid volume ; OS/BS ; osteoid surface ; ES/BS ; eroded surfaces ; N.Oc/B.Pm and N.Oc/B.Ar ; number of osteoclast as reported to bone perimeter and area ; respectively ; Oc.S/BS ; active eroded surface ; FFN and FFC ; nuclear and cellular form factor ; N/C ; nuclear&ndash ; cytoplasmic ratio ; SM ; skeletal metastasis
• 刊名：Pathology - Research and Practice
• 出版年：March, 2014
• 年：2014
• 卷：210
• 期：3
• 页码：135-141
• 全文大小：1953 K

文摘

We examined the effects of vitamin E supplementation (VES) on osteoclast (OC) resorbing activity and cytomorphometry in Walker 256/B tumor osteolytic rats.

Twenty-four aged male rats were randomized into 3 groups: 6 were sham operated; 9 were injected in the right hind limb with Walker 256/B cells (W256 group); and 9 were injected as above and supplemented with VE (45 mg/kg BW) (W256VE group). Twenty days later, bone mass (BV/TV) and some microarchitectural parameters were assessed. Some histodynamic parameters, cellular and nuclear form factors (FF_C and FF_N), and nuclear-cytoplasmic ratio (N/C) of OC were measured for each group.

W256 group exhibited osteolytic lesions in the operated femora. Walker 256/B induced trabecular perforation and decreased BV/TV associated with significant increases in OC numbering (N.Oc/B.Ar and Oc.N/B.Pm) and activity (ES/BS and Oc.S/BS). While FF_N remain unchanged, the FF_C and N/C ratio increased in the W256 group. W256VE showed less osteolytic lesions. Moreover, disruption of bone microarchitecture and OC activity in W256VE group decreased. VES reduced the malignant Walker 256/B-induced enhanced OC resorbing activity with cytoinhibition rate reaching 41%.

The protective effect of VE may be due to its modulation of OC cytomorphometry and subsequently their activity.