C2c1-sgRNA Complex Structure Reveals RNA-Guided DNA Cleavage Mechanism

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• 作者：Liang Liu¹ ; Peng Chen¹ ; ² ; Min Wang¹ ; Xueyan Li¹ ; ² ; Jiuyu Wang¹ ; Maolu Yin¹ ; ² ; Yanli Wang¹ ; ² ; ³ ; ⁴ ; ^{ylwang@ibp.ac.cn}
• 关键词：CRISPR-Cas system ; C2c1 ; crystal structure ; DNA endonuclease ; genome-editing
• 刊名：Molecular Cell
• 出版年：2017
• 出版时间：19 January 2017
• 年：2017
• 卷：65
• 期：2
• 页码：310-322
• 全文大小：6049 K
• 卷排序：65

文摘

Crystal structure of Alicyclobacillus acidoterrestris C2c1-sgRNA complex Mechanistic insights into dual-RNA-guided, C2c1-catalyzed, staggered dsDNA breaks High mismatch sensitivity of C2c1 reduces off-target cleavage Join 2/4-truncated sgRNA-guided DNA cleavage similar to that of wild-type sgRNA