Visualizing double-stranded RNA distribution and dynamics in living cells by dsRNA binding-dependent fluorescence complementation
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- 作者：Xiaofei Chenga ; b ; Ping Denga ; Hongguang Cuia ; Aiming Wanga ; aiming.wang@agr.gc.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：Live-cell imaging ; Double-stranded RNA ; RNA binding protein ; Fluorescent protein ; BiFC ; Positive-sense RNA virus ; Viral replication complex ; SGS3 ; RDR6 ; D-bodies
- 刊名：Virology
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：485
- 期：Complete
- 页码：439-451
- 全文大小：5660 K
文摘
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A live-cell imaging system was developed for visualizing dsRNA in vivo.
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It uses dsRNA binding proteins fused with two halves of a fluorescent protein.
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Binding to a common dsRNA enables the reporter to become fluorescent.
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The system can efficiently monitor viral RNA replication in living cells.