Genome-wide RNA-seq and ChIP-seq reveal Linc-YY1 function in regulating YY1/PRC2 activity during skeletal myogenesis

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• 作者：Kun Sun<sup>asup><sup> ; sup><sup>bsup> ; Liang Zhou<sup>bsup> ; Yu Zhao<sup>bsup> ; Huating Wang<sup>bsup><sup> ; sup><sup>csup><sup> ; sup><sup>huating.wang@cuhk.edu.hk" class="auth_mail" title="E-mail the corresponding authorsup> ; Hao Sun<sup>asup><sup> ; sup><sup>bsup><sup> ; sup><sup>haosun@cuhk.edu.hk" class="auth_mail" title="E-mail the corresponding authorsup>
• 关键词：Linc-YY1 ; Myogenesis ; RNA-seq ; ChIP-seq ; C2C12 cells
• 刊名：Genomics Data
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：7
• 期：Complete
• 页码：247-249
• 全文大小：211 K

文摘

Little is known how lincRNAs are involved in skeletal myogenesis. Here we describe the discovery and functional annotation of Linc-YY1, a novel lincRNA originating from the promoter of the transcription factor (TF) Yin Yang 1 (YY1). Starting from whole transcriptome shotgun sequencing (a.k.a. RNA-seq) data from muscle C2C12 cells, a series of bioinformatics analysis was applied towards the identification of hundreds of high-confidence novel lincRNAs. Genome-wide approaches were then employed to demonstrate that Linc-YY1 functions to promote myogenesis through associating with YY1 and regulating YY1/PRC2 transcriptional activity in trans. Here we describe the details of the ChIP-seq, RNA-seq experiments, and data analysis procedures associated with the study published by Zhou and colleagues in the Nature Communications Journal in 2015 Zhou et al. (2015) <span id="bbb0005">[1]span>. The data was deposited on NCBI's Gene Expression Omnibus (GEO, <span id="ir0005" class="interref" data-locatorType="url" data-locatorKey="http://www-ncbi-nlm-nih-gov.library.gcu.edu:2048/geo/">http://www-ncbi-nlm-nih-gov.library.gcu.edu:2048/geo/span>) with accession number GSE74049.