Screening Psychrophilic Fungi of Cellulose Degradation and Characteristic of Enzyme Production

详细信息    查看全文

• 作者：Wang Da-qing^a ; ^* ; ^{wangdaqing511@163.com} ; Jin Wen-ran^b ; Sun Tai-peng^b ; Meng Yu-tian^b ; Zhao Wei^b ; ^{niersi7105@sina.com} ; Wang Hong-yan^b
• 关键词：low temperature ; cellulose-degrading fungi ; screening ; enzyme activity
• 刊名：Journal of Northeast Agricultural University (English Edition)
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：23
• 期：2
• 页码：20-27
• 全文大小：614 K
• 卷排序：23

文摘

A fungus (WR-C1) decomposed cellulose was isolated from a hypothermal litter layer using Congo red medium as the preliminary screening culture medium and then using a filter as the secondary screening medium at low temperature. The experiment showed that the weight loss rate of filter paper on the 15th days could reach 30.69%. A morphologic and ITS gene sequence analysis suggested that CF-C1 was Cladosporium. We mainly studied the effects of culture time, inoculation amount, initial pH and different sources of carbon, nitrogen and inorganic salt on the cellulase production of strain WR-C1. Under optimum cultural condition, the highest value of WR-C1 enzyme production and filter paper enzyme were 3.27 U · mL−1 and 0.51 U · mL−1.