Improving the productivity of S-adenosyl-l-methionine by metabolic engineering in an industrial Saccharomyces cerevisiae strain
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- 作者：Weijun Zhaoa ; Baojian Hanga ; Xiangcheng Zhub ; seanzhu1996@aliyun.com" class="auth_mail" title="E-mail the corresponding author (Dr. ; Associate professor) ; Ri Wanga ; Minjie Shena ; Lei Huanga ; Zhinan Xua ; znxu@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author (Dr. ; Professor)
- 关键词：S-Adenosyl-l-methionine ; Saccharomyces cerevisiae ; SAM2 ; SPE2 ; GLC3
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 October 2016
- 年：2016
- 卷：236
- 期：Complete
- 页码：64-70
- 全文大小：1178 K
文摘
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The deletion of several key genes for glycogen synthesis or in SAM decarboxy pathway could greatly improve the SAM production in S. cerevisiae.
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Combined with the overexpression of SAM2 and pseudo-exponential feeding of glucose, a high SAM productivity (12.47 g/L) was achieved in the bench-top fermenters.
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It was found that low ergosterol content in ZJU001, which had been evolved long time, was responsible for no change of SAM production after the disruption of ERG4 or ERG6.