Biodegradation of nicosulfuron by a novel Alcaligenes faecalis strain ZWS11

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• 作者：Weisong Zhao ^{zhaoweisong1985@163.com" class="auth_mail" title="E-mail the corresponding author} ; Chen Wang ; Li Xu ; Chunqing Zhao ; Hongwu Liang ; Lihong Qiu ; ^{lihongqiuyang@126.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Biodegradation ; Nicosulfuron ; Alcaligenes faecalis ; Metabolite
• 刊名：Journal of Environmental Sciences
• 出版年：2015
• 出版时间：1 September 2015
• 年：2015
• 卷：35
• 期：Complete
• 页码：151-162
• 全文大小：5568 K

文摘

A bacterial strain ZWS11 was isolated from sulfonylurea herbicide-contaminated farmland soil and identified as a potential nicosulfuron-degrading bacterium. Based on morphological and physicochemical characterization of the bacterium and phylogenetic analysis of the 16S rRNA sequence, strain ZWS11 was identified as Alcaligenes faecalis. The effects of the initial concentration of nicosulfuron, inoculation volume, and medium pH on degradation of nicosulfuron were investigated. Strain ZWS11 could degrade 80.56% of the initial nicosulfuron supplemented at 500.0 mg/L under the conditions of pH 7.0, 180 r/min and 30°C after incubation for 6 days. Strain ZWS11 was also capable of degrading rimsulfuron, tribenuron-methyl and thifensulfuron-methyl. Four metabolites from biodegradation of nicosulfuron were identified, which were 2-aminosulfonyl-N, N-dimethylnicotinamide (M1), 4, 6-dihydroxypyrimidine (M2), 2-amino-4, 6-dimethoxypyrimidine (M3) and 2-(1-(4,6-dimethoxy-pyrimidin-2-yl)-ureido)-N,N-dimethyl-nicotinamide (M4). Among the metabolites detected, M2 was reported for the first time. Possible biodegradation pathways of nicosulfuron by strain ZWS11 were proposed. The degradation proceeded mainly via cleavage of the sulfonylurea bridge, O-dealkylation, and contraction of the sulfonylurea bridge by elimination of a sulfur dioxide group. The results provide valuable information for degradation of nicosulfuron in contaminated environments.