Stimulation of bone growth following zinc incorporation into biomaterials

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• 作者：Yuqin Qiao^a ; ¹ ; Wenjie Zhang^b ; ^c ; ¹ ; Peng Tian^a ; Fanhao Meng^a ; Hongqin Zhu^a ; Xinquan Jiang^b ; ^c ; ^{xinquanjiang@aliyun.com" class="auth_mail} ; Xuanyong Liu^a ; ^{xyliu@mail.sic.ac.cn" class="auth_mail} ; Paul K. Chu^d
• 关键词：Zinc ; Titanium ; Plasma immersion ion implantation and deposition ; Osteogenic activity ; Osseointegration
• 刊名：Biomaterials
• 出版年：August 2014
• 年：2014
• 卷：35
• 期：25
• 页码：6882-6897
• 全文大小：7354 K

文摘

Rapid development of zinc biology has broadened the applications of Zn-incorporated biomaterials to tissue engineering but also raised concerns about the long-term safety of released Zn²⁺ ions. Clinical success hinges on the amount of incorporated zinc and subsequent optimized release sufficient to stimulate osseointegration. In this study, zinc is incorporated into the sub-surface of TiO₂ coatings by plasma immersion ion implantation and deposition (PIII&D). The Zn-implanted coatings show significant improvement compared to the “bulk-doped” coatings prepared by plasma electrolyte oxidation in terms of osteogenesis in vitro and in vivo. Molecular and cellular osteogenic activities demonstrate that rBMSCs cultured on the Zn-implanted coatings have higher ALP activity and up-regulated osteogenic-related genes (OCN, Col-I, ALP, Runx2) compared to the bulk-doped Zn coatings and controls. In vivo osseointegration studies conducted for 12 weeks on the rat model show early-stage new bone formation and the bone contact ratio (12 week) on the Zn-implanted coating is larger. The ZnT1 and ZIP1 gene expression studies demonstrate that the Zn-implanted coatings can better stimulate bone growth with reduced Zn release than those doped with zinc throughout the coatings.