Highly selective detection of glutathione using a NIP/Cu²⁺ complex fluorescent probe

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• 作者：Wenrui Liang ; Zhi Zhao ; Yang Zhang ; Qiusheng Wang ; ^{wangqsh@tjut.edu.cn} ; ^{qiushengwang_cn@yahoo.com.cn} ; Xin Zhao ; Jie Ouyang
• 关键词：Imidazole ; Fluorescent probes ; Glutathione ; Excited-state Intramolecular Proton Transfer
• 刊名：Journal of Luminescence
• 出版年：2012
• 出版时间：May, 2012
• 年：2012
• 卷：132
• 期：5
• 页码：1160-1165
• 全文大小：492 K

文摘

A novel fluorescent compound, 4-(trimethyl ammonium chloride)acetamide-2-(1H-naphtho[2,3-d]imidazol-2-yl)phenol (TMACA-NIP), was synthesized and used as a fluorescent probe for detecting glutathione reduced (GSH). The new NIP-based probe exhibited high fluorescence in water, which was quenched during the presence of copper (II) due to the complexation between TMACA-NIP and Cu²⁺. But after adding GSH into the TMACA-NIP and Cu²⁺ system, the fluorescence of TMACA-NIP was recovered because the binding force between GSH and Cu²⁺ is stronger than that between TMACA-NIP and Cu²⁺, which destroys the equilibrium between NIP and copper (II) ions and releases the fluorescence probe of TMACA-NIP. This three-component competing system of NIP/Cu²⁺/GSH can be used to detect GSH simply and rapidly.