Waste fish oil biodiesel instead of diesel decreased the cycle-to-cycle variations.

WFO had about 2.92% more gross thermal efficiency when compared with the diesel.

Combustion loss was about 15.2% for B100 and 15.72% for pure diesel fuel.

WFO as alternative fuel showed the huge potential to reduce CO and UHC emissions.

Biodiesel showed about 12.8% higher NOx averagely throughout all engine loads.